Merging two 10x single cell datasets

I was going to write a post on using the Seurat alignment method as a batch correction tool but as it turned out the two datasets that I chose didn’t seem to have strong batch effects! I heard about the alignment method sometime last year but was motivated to try it out after listening to…

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Getting started with Seurat

Updated 2018 April 11th This post follows the Peripheral Blood Mononuclear Cells (PBMCs) tutorial for 2,700 single cells. It was written while I was going through the tutorial and contains my notes. The dataset for this tutorial can be downloaded from the 10X Genomics dataset page but it is also hosted on Amazon (see below)….

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